Assume that one conducted a typical cloning experiment using puc18, transformed an appropriate host bacterial strain (one carrying the lacz complementing region), and plated the bacteria on an appropriate x-gal medium. blue and white colonies appeared. which of the two types of colonies, blue or white, would most likely contain the recombinant puc18? explain your answer.

The pUC18 refers to a polylinker that is featured by enhanced multiple restriction locations. The enhanced number of restriction sites would upsurge the chance of the target DNA sequence being presented into the plasmid. If the sequence of the DNA is administered efficiently and obstructs the sequence of the LacZ gene in the restriction site, the bacteria will not be able to generate B galactosidase that cleaves X-gal.  

When the X-gal in the medium does not get cleaved, the colonies become white. In case, if the DNA was not effectively presented into the host DNA and the LacZ gene can generate B galactosidase, X-gal will get cleaved and the colonies will be blue.  

As the white colonies will be the hosts comprising the recombinant DNA, and pUC18 enhances the chance of DNA getting inserted, the white colonies will most probably comprise the recombinant pUC18.  

picture a world where computers are building made-to-order personalized robotic arms and synthetic organs, drones are circulating above mars! due to the rapid growth of technology, the future has never been more unpredictable, and it presents a huge challenge in education: we are teaching students how to work in a future that we have never been unsure of. it is widely predicted that in eight years’ time, there will be more jobs in the fields of science, mathematics, technology and engineering than in any other discipline. and, as the technology continues to develop, it will soon become clear that addressing social problems will involve more innovative thinking, and analytical and technical knowledge than ever before. undeniably, there are no subjects that encourage innovative and creative thinking, deep thought, problem solving and independence more than the stem subjects. while it is widely commented on that there is a need to reimagine the ways stem subjects are taught in schools by embracing a more interactive and innovative approach to appeal to more students, it should come as no surprise that some of the most reputable educational organizations in the world are already implementing changes to embrace stem. harvard, for example, announced in 2014 that they were increasing their computer science faculty by over 50% and has since introduced computer science as a core subject for all freshmen. the urgency of supporting stem subjects at a young age is becoming more relevant than ever, as adopting a keen interest in the curricula needs to happen early. however, beyond the age issue is one of gender.

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