What is the impact of using irony in a story's ending

When you use irony to close off a story it is usually used to impact a cold and non-light-hearted ending. It is used to show cruelty and coldness instead of ending the story with light. The brother's Grimm did this a lot because their stories were dark and had less of a "touchy" feel. Hope this helps! :)

Explanation:

this protocol provides a detailed procedure for agarose-based ip to purify and enrich the protein of interest.