Biology, 28.11.2019 02:31 valerieaj113
For her undergraduate project, jessica studied an enzyme that catalyzes the reaction . for substrate a, she determined that km = 4.5 μm and kcat = 35 min–1. jessica graduated and her project has been passed on to you. unfortunately, jessica was so busy that she sometimes forgot to record all of the details of an assay in her lab notebook. your mentor suggests that you try to back calculate some of the missing concentration values. assume that the enzyme follows michaelis-menten kinetics. a.) in one experiment where [a] = 5 mm, she found that the initial velocity, v0 was 334 nm min–1. what was the [et] she used in this experiment? b.) in another experiment with [et] = 0.6 μm, she found that v0 = 3 μm min–1. what was the [a] she used in this experiment? c.) the compound z was found to be a very strong competitive inhibitor of the enzyme, with an α of 10. in an experiment with the same [et] as in part (a), but a different [a], an amount of z is added that reduces the rate v0 to 167 nm min–1. what was the [a] in this experiment? d.) a measure of the catalytic efficiency of an enzyme is the initial slope of the michaelis-menten curve at low [s], the second order rate constant kcat/km. a "perfect" enzyme, limited only by diffusion, would have a value of 108 or 109 m–1s–1. calculate kcat/km for this enzyme.
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For her undergraduate project, jessica studied an enzyme that catalyzes the reaction . for substrate...
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