An organism grown in nutrient rich broth to high turbidity always produces a blue pigment. when a large inoculum is transferred to a nutrient rich agar plate, it also appears blue. a researcher noticed that this never happens when very small colonies are grown on low nutrient agar plates, however. what is the most plausible conclusion? the strong gradient from very high to low nutrient bioavailability induces production of the blue metabolite. production of the blue pigment production is linked to quorum sensing. only high nutrient conditions provide enough energy for cells to produce this secondary metabolite that appears blue. large populations enabled the differentiation of a subpopulation of cells that created the blue pigment. 2 points

Production of blue pigment is linked to quorum sensing.

Explanation:

This blue pigment formation in the bacteria is triggered and entirely linked with quorum sensing control method. Quorum sensing is the process by which the pigmented bacteria could the detect on sense the presence of cells in large density. Here the organism under consideration with the help of accumulating the molecules that signals the presence of higher cell density, henceforth the blue pigments being the signalling molecules are accumulated and hence the blue pigmentation.

americans began to notice growing differences between the northern and southern sections of the country. there were more people living in cities in the north, which made it more urban. more people lived on farms in the south, which made it more rural. while many people were farmers in the northern states, there were now other ways to make a living, such as working in mechanical cotton gins factories manufacturing cloth, tools and other goods. people in the north supported high tariffs on goods imported from europe because the extra cost encouraged people to “buy american” instead. in the southern states, planters did not like tariffs because they sold most of their production to europe and bought their tools from there as well. southerners believed the protective tariff was something that only northern factory owners keep their profits high. so the southern politicians came up with an idea. they would opt out. they would simply ignore the protective tariff. it was ‘null and void’ in south carolina. the state would not collect the taxes on goods imported into south carolina, no matter what the president said they had to do. this decision led to the nullification crisis.