In the cloning procedure we used in the lab, it is possible, albeit unlikely, for multiple inserts to form a concatamer and be cloned into a single expression vector. if three egfp inserts were cloned into a single pet-41a expression vector, would egfp be expressed (following iptg induction), and if so, would these colonies have more fluorescence compared to clones that have one copy of the egfp gene (following iptg induction)

The specific heat of ice is 0.5 calories/gram°c. 20 grams of ice will require ll calories to raise the temperature 1°c. 05

Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also known as β-lactamase), an enzyme present in some resistant bacteria. the mass of this enzyme in staphylococcus aureus is 29.6 kd. the amount of penicillin hydrolyzed in 1 minute in a 10-ml solution containing 10-9 g of purified penicillinase was measured as a function of the concentration of penicillin. assume that the concentration of penicillin does not change appreciably during the assay. penicillin(μm) amount hydrolyzed(nmol) 1 0.11 3 0.25 5 0.34 10 0.45 30 0.58 50 0.61 (a) plot v0 versus [s] and 1/v0 versus 1/[s] for these data. does penicillinase appear to obey michaelis-menten kinetics? if so, what is the value of km? (b) what is the value of vmax? (c) what is the turnover number of penicillinase under these experimental conditions? assume one active site per enzyme molecule.

Which type of mutation occurs in reproductive cells and can be passed to offspring? somatic mutation germline mutation point mutation frameshift mutation