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Biology, 16.10.2019 04:30 dnation94

Why does double-stranded dna with a high a-t content have a lower transition temperature, tm, than double-stranded dna with a high c-g content? the fewer h-bonds for a-t-rich dsdna cause its two strands to stick together less well than g-c-rich dsdna. a-t-rich dsdna requires more energy to separate into single strands than g-c rich dsdna. elevated temperature breaks the backbone bonds between c and g bases less easily than they break the backbone bonds between a and t bases. purine bases are more susceptible to hydrolysis at high temperature than are pyrimidine bases.

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