What is the purpose for using a hydropathy plot?
a. predict whether a protein is soluble
b. predict the quaternary structure of a membrane protein
c. predict secondary structure of a membrane protein
d. determine the true molecular weight of a membrane protein
e. predict whether a given protein sequence contains membrane-spanning segment

The correct answer will be option-E.

Explanation:

A Hydropathy plot is a visual representation of the hydrophobic nature of a protein over a plot. The plot allows visualizing the hydrophobicity in a sequence of the peptide.

The plot is prepared on the basis of a hydrophobic scale of 20 different amino acids which is used to determine the membrane-spanning regions of membrane-bound proteins and hydrophobic interior portions of globular proteins.

Thus, option- E is the correct option.

Hydropathy Plot. Hydropathy plots allow for the visualization of

hydrophobicity over the length of a peptide sequence. ... Such plots are useful in determining the hydrophobic interior portions of globular proteins as well as determining membrane spanning regions of membrane bound proteins.

Hydropathy plots allow for the visualization of hydrophobicity over the length of a peptide sequence. A hydropathy scale which is based on the hydrophobic and hydrophilic properties of the 20 amino acids is used. A moving "window" determines the summed hydropathy at each point in the sequence (Y coordinate). These sums are then plotted against their respective positions (X coordinate). Such plots are useful in determining the hydrophobic interior portions of globular proteins as well as determining membrane spanning regions of membrane bound proteins.

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