Describe the steps in transcription that require complementary base pairing.

Explanation:

Transcription takes place in nucleus in the presence of the enzyme RNA polymerase.

During transcription, the code present in DNA gets transcribed into the code in mRNA.  (messenger RNA)

RNA even though is a single stranded shows base pairing.

The pre-mRNA gets created from the DNA.

DNA has two strands, one of these strands acts as coding and another non coding strand.

The DNA shows base pairing with AGTC.   [A-Adenine, G- Guanine, T-Thymine and C- Cytosine]

Whereas mRNA base pairing with AGUC.  [ U- Uracil ]

Here in mRNA, instead of thymine, uracil gets paired.

According to base pairing of coding strand/sense strand of DNA, complementary bases pairs in 5'>3' direction in mRNA.

The mRNA created looks exactly like the non-coding strand/sense strand of DNA except instead of thymine, uracil gets replaced.

The pre-RNA undergoes splicing, capping and tailing to form mature mRNA.

the cell theory states that all living things including animals, as well as plants, are made of cells.

this protocol describes a detailed procedure for intracellular flow cytometry staining.