Order the steps that occur as a protein is synthesized within a cell and finally excreted for use outside of the cellitemmove bottonrough er golgi bodycell membranenucleusmrnaribosome

The correct answer is:

Nucleus, mRNA, Rough ER, Ribosome, Golgi Body, Cell Membrane.

Explanation:

Protein synthesis is separated into two steps which are transcription and translation. Transcription happens in the nucleus and it includes the encoding of the mRNA with the genetic information in the DNA. Translation begins when the mRNA transfer from the nucleus to the cytoplasm. In the cytoplasm, the protein assembly takes place inside the ribosomes after which they are transferred to the rough endoplasmic reticulum for adjustments.

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.