are similar to viroids
are only needed once
kill viruses
stimulate an immune response

Stimulate an immune response

Explanation:

The vaccine is a preparation that contains a weakened or killed form of microorganism (or part of them) that stimulates the immune system to produce memory antibodies to recognize that microorganism in the future. After the body produces the memory antibodies, if a person is in contact with that microorganism the body recognized them as “strange” and the body response to killing them is faster before the infection. In some cases is necessary to apply various doses of the vaccine in a certain time to maintain the memory antibodies.

in situ hybridization has been widely used for research applications, including clinical cytogenetics, gene mapping, tumor biology and studies of chromosome evolution.