Aflask of 100 ml growth medium is inoculated with 500 stationary-phase bacteria and placed in optimum incubation condition.
they are incubated for 6 hours and are observed to be in lag phase for the first hour before they start growing exponentially.
at the end of the 6 hours, the bacteria density is measured by serial dilution followed by plating. it is found to be 1.28 x 103 cfu/ml
a) how many bacteria are there in the culture in total after 6 hours?
b) how many times have the bacteria doubled during this time?
c) using the answers to (a) and (b), what is the generation time?

Atp is is our little "pocket" of energy. of course, if you have too little, then it means you have too little energy, and you'll pass out. the way to not have enough, is to have no energy sources (glucose, fats, proteins). you can't have too many atp molecules. cycles like the krebs cycle produce it, but if you have too much of any substrate (or too little for that matter), the cycle stops and atp is no longer made (it stops until substrate levels are such that the process can go on). in any case, this is unlikely since atp is constantly hydrolyzed and remade, even while you're asleep. you won't have too much. if you have zero, you are dead or dying.