Which of the following is not one of the three basic steps involved in enzyme activity? which of the following is not one of the three basic steps involved in enzyme activity? the enzyme's active site binds to the substrate(s) on which it acts, temporarily forming an enzyme-substrate complex. the substrate absorbs chemical energy from the enzyme after binding to its active site. the enzyme releases the product(s) of the reaction. the enzyme-substrate complex undergoes internal rearrangements that form the product(s).