Match the staining techniques with their purpose.
a negative stain such as india ink or congo red.
b the gram stain
c the ziehl-neelsen acid-fast stain
d simple stain with a basic dye
e the schaeffer-fulton endospore stain

stain microbes a bright color to make it easier to see them in bright field microscopy.
identify bacillus or clostridium species, such as the causative agents of anthrax, botulism, tetanus and gangrene.
divide bacteria into two groups based on cell wall structure (thick vs. thin).
identify bacteria with waxy cell walls such as: mycobacterium tuberculosis (the cause of tb).
look for the presence of a capsule.

A Negative stain such as India ink or Congo red-Look for the presence of a capsule.

This stain method is usually used to stain the area around the microorganism.

B The Gram stain-Divide bacteria into two groups based on cell wall structure (thick vs. thin).  

This is type of differential staining used to distinguish organisms based on their staining properties. Gram + and Gram- bacteria stain different because of different cell wall structure.

C The Ziehl-Neelsen Acid-fast stain- Identify bacteria with waxy cell walls such as: Mycobacterium tuberculosis (the cause of TB).  

This is also differential staining method which uses heat and phenol to derive dye into the cells with lipid-rich walls.

D Simple stain with a basic dye-Stain microbes a bright color to make it easier to see them in bright field microscopy.

One dye is used in simple staining in order to determinate the size, shape and arrangement of the cells.

E The Schaeffer-Fulton Endospore stain-Identify Bacillus or Clostridium species, such as the causative agents of anthrax, botulism, tetanus and gangrene.  

This is a special type of staining only used for the bacteria that can form endospores. Bacteria are first treated with heat and then with malachite green, which is very strong stain that can penetrate endospores.

creative diagnostics provides a protocol for total protein extraction based on ripa which is widely used.