When the stains are eluted crystal violet is retained near the top of the soil column, whereas eosin y is retained throughout the length of the column?

Crystal Violet retained towards the top of the soil column while Eosin Y was seen throughout the length of  column because they have different charges. Cation exchange and polarity, have much to do with each dye,and each dye went to different places. This process depends on binding sites and the charges of the soil, which is also related to water-holding capacities. Since Crystal Violet was eager to accept a hydrogen molecule and our soil had a negative charge,so Crystal Violet remained towards the top and leave the site positively charged.Polar Eosin Y has a negative net charge, like our soil, and diffused more rapidly through the length of the soil column.

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