Wildfires have both negative and positive effects on an ecosystem. which is anegative effect of wildfire on any ecosystem?

The wildfire decimates abiotic and biotic factors in a populations' habitat hence  reducing the resources required to sustain the entire population. This devastates the population numbers due to increased competition for reduced  resources. Wildfire especially decimates primary producers hence affecting the organisms up  the food chain.

the answer to your question is b

this protocol provides a detailed procedure for agarose-based ip to purify and enrich the protein of interest.