Do aquaporins require a living cell in order to function? explain

No, if you can reconstitute an aquaporin in a membrane it will function fine. Aquaporins that actively transport water will not function, but many aquaporins are passive transporters. All the above being said, we are still not close enough to use aquaporins as well as biology does.

step 1: nitrogen fixation- conversion of atmospheric no₂ into forms useful to plants by lightning, bacteria, and cyanobacteria.

step 2: nitrification-process of converting ammonia to nitrite or nitrate.

step 3: assimilation-plant roots absorb new form of nitrogen.

step 4: ammonification-conversion of organic nitrogen to ammonia by bacteria involved in decomposition.

step 5: denitrification-bacteria turns the decomposed matter back into n₂o, which is released into atmosphere; repeat cycle.