How is the brain an experimental animal preserved and prepared for sectioning?

In order to get detailed histological data of the brain high-quality histological sections and reliable histological processing must be done. There are a few steps in a process of preparation tissue before sectioning:

•Fixation - (formaldehyde and formalin are the most commonly used  fixatives). This step is necessary to preserve the tissue sample in a state as close to that during life.

•Dehydration - by using high concentrations of alcohol ( 60% ethanol in water) Ths step is for removing excess fluid.

•Clearing – (xyrene, benzene , chloroform) Process of removing the dehydrating agent.

•Embedding (paraffin) The step of putting the tissue in a medium to allow thin slices to be cut cleanly. It provides rigid support to the tissue.

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