What can be said about all the dna molecules in a single sanger sequencing reaction mix?

Is a method of dna sequencing first commercialized by applied biosystems, based on the selective incorporation of chain-terminating dideoxynucleotides by dna polymerase during in vitro dna replication.[1][2] developed by frederick sanger and colleagues in 1977, it was the most widely used sequencing method for approximately 40 years.