Why are controls not needed in the arthropods experiment described in the study guide

Amylase becomes denatured at a temperature of 80°c. during an experiment to study the effect of varyingtemperature on enzyme activity, amylase’s reactivity with starch was measured at body temperature (37°c), andthen again at an increased temperature of 42°c. how would this increase in temperature affect the experiment

Which statement about relative potential energy of electrons is correct? a. an electron in the 2 p orbital of the second electron shell has more potential energy than an electron in the 2 s orbital of the second electron shell. b. an electron in the 2 p orbital of the second electron shell has more potential energy than an electron in the 3 p orbital of the third electron shell. c. an electron in the 3 p orbital of the third electron shell has more potential energy than an electron in the 2 p orbital of the second electron shell.

How do all types of diffusion/passive transport actually ‘work’ without using even the smallest amount of cellular energy?